Royal Life sciences Pvt Ltd
Products

Royal is a sole distributor of MIDI Sherlock® Microbial Identification System (MIS) for Bahrain, Bahrain, Burindi, Democratic Republic of Congo, Ethiopia, India, Iraq, Jordan, Kenya, Kuwait, Lebanon, Malawi, Oman, Qatar, Rwanda, Saudi Arabia, South Sudan, Sri Lanka, Tanzania, Uganda, United Arab Emirates,Yemen, and Zambia We are Value-added reseller of Agilent Technologies to provide single-window solutions and turnkey Project for establishing MIDI Sherlock Lab with Online /Offline support by providing AMC. We are authorized one stop shop for MIDI Sherlock to supply uninterrupted consumables & spares.

Our Products are:


Microbial Identification System

If you need to know simply, inexpensively, and right now - then you need to know MIDI.

The Sherlock MIS was launched in 1991 and represents the only commercially available microbial identification system that uses Cellular Fatty Analysis by Gas Chromatography (GC-FAME).  This technology has been used for more than 50 years as a rapid, accurate, inexpensive and easy-to-use method for bacterial and yeast identification of over 1,500 microbial species

sherlock-microbial-identification-system-mis

The Sherlock MIS is recognized by the U.S. CDC for the identification of aerobic bacteria and is AOAC INTERNATIONAL cleared for the identification of Bacillus anthracis, the anthrax pathogen. The Sherlock MIS was created by microbiologists for microbiologists, so no GC experience is necessary.

What makes the Sherlock MIS unique is that all microbes are analyzed using the same procedure, with no offline tests, gram stains or biochemical cards required. The system is FDA 21CFR Part 11 compliant and validation packages are available for Bio/Pharma QC customers.  

In addition to microbial ID, the Sherlock MIS can be used to analyze fatty acids and related compounds from soil (eg. PLFA), algal biofuels and omega-3 fatty acids, thereby increasing the systems utility for researchers.

Key Features of the Sherlock MIS include:

  • Rapid ID in less than 15 minutes.
  • Inexpensive per sample cost.
  • Automated analysis and naming.
  • Throughput over 100 samples per day.
  • No biochemical kits or cards.
  • Comprehensive data analysis tools.
  • Proven reliability and warranty.
  • Combine with DNA Sequencing Results
fame_procedure

Fatty acid methyl ester analysis by gas chromatography (GC-FAME) has been used for over 50 years to identify microbes in environmental and clinical settings. FAME identification is a dynamic test of the organism. It is specific to growth conditions, including the media and temperature used. Different libraries are available for different growth conditions and custom libraries can be created based on the customer's unique environment. In addition to identification, FAME analysis is often sensitive enough to give strain level information for a sample.



Sherlock DNA Sequencing Software

Sherlock DNA Sequencing Software is something that we export, trade and supply. This software is used for analyzing and identifying fatty acid methyl ester (FAME) as well as microbes that are based on 16S/28S rRNA gene sequence. Sherlock DNA starts when DNA sequencer leaves off having textual consensus sequencing. The best advantage of this software is that it can be used with sequencers sourced from any of the vendors. 16S identification is a technique that is static in nature and provides results without compromising on the growth conditions. 16S can be reproduced with slight variations of running. The 16S gene is conserved at species level and thus it gets sequence. This results in offering almost same results in cases of strains in identical species. One can also rely on these s16 for identification of strain-level in some species, whereas FAME can also be a good choice. For some specific generation one among the two technologies available can obtain best results.

sherlock-dna-sequencing-software

For detailed information of any sample the Sherlock Software can be used for processing 16S and Fame identification correlating the results in single "polyphasic" reports. Fame and 16S both are orthogonal techniques (techniques that rely on independent information from the samples), where the two FAME and 16S agrees to each other providing overall confidence with improved results.

One can compare the samples with one another using Sherlock DNA with result that is displayed in a number of forms. The sequence alignment tools and familiar neighbor joining trees are the two forms of results. A new type display is developed by MIDI.  Accurate Root Neighbor Joining Tree is a form of result finding that compares an unknown sample in between the group of samples.

Key driving features of these products include:

  • Full integration with MIDI's Sherlock Software
  • Validated libraries for bacterial ID (500bp and full gene 16S)
  • Combined reporting of DNA-FAME results
  • DNA analysis tools for direct comparison of DNA samples
  • Any DNA sequencer vendor can be used
  • Validated libraries for fungi/yeast ID (500bp 28S)
  • Custom DNA library development tools