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Our

Services

 

Complete Microbial and R & D Services.

We don't just sell products and services.... Royal offers solutions to research problems.
"To the rest of the world these are samples, to us they are a scientist's lifetime work, a lab's mission, a company's breakthrough. We are obsessed with sample integrity. Our services with innovative inventory technologies make us globally famous."

Our organization is instrumental in offering a wide range of Microbial analysis and R & D services. We are equipped with a sound infrastructure, which enables us to perform these services efficiently. The professionals of our team are experienced enough to conduct various microbial identification processes. We offer these services keeping in mind the industrial standards and specifications of the clients. Our service range includes Fame Analysis, PLFA (Phospholipid Fatty Acid), Biochemical Characterization of Microbe and DNA Sequencing and related research services.

Features:

  • Accurate identification
  • Reliable
  • Timely and customized services.

PLFA (Phospholipid Fatty Acid)

PLFA is a phenotypic approach to assess the microbial community structure in the sample. PLFA analysis is based on the extraction of 'signature' lipid biomarkers from the cell membranes of the microbes and conversion of the lipids to their respective FAMEs.

PLFA analysis is an extremely robust technology with a long history that has been applied to nearly every specialty in the microbiology field including:

  • Bioremediation Assessment
  • Wastewater Treatment
  • Drinking water Treatment
  • Microbial Induced Corrosion
  • Indoor Air Quality
  • Bio Reactors.

DNA Sequencing

We offer a wide range of services related to DNA Sequencing. DNA Sequencing is a processes of evaluation of the nucleotide bases—adenine, cytosine, guanine and thymine—in a molecule of DNA. DNA sequence is very important for basic biological researches and is required in various areas like diagnostic, biotechnology or forensic biology. Number of DNA sequencing technologies has been developed to perform sequencing. Our professionals use FAME analysis, DNA sequencing and microscopic analysis for identifications, which make us the preferred choice of many clients. The mentioned tools are combined to form a well-rounded and exact identification system.

Our DNA sequencing services include:

  • High through-put sequencing of 16 S rDNA/28 S rRNA and identification of microbes

Features:

  • Utilization of modern tools and techniques.
  • Experienced professionals perform these services.
  • Reasonable prices.

Biochemical Characterization

Biochemical analysis techniques refer to a set of methods, assays and procedures that enable researchers to analyze the substances found in living organisms and the chemical reactions underlying life processes. It looks at the levels of disturbances and can be used to detect several different metabolic conditions. This characterization helps us to develop an understanding of the biochemical differences in unknown bacteria.We offer over 35 tests:

  • Carbohydrates
  • Nitrate
  • Oxidase
  • Urease
  • Citrate
  • Catalase
  • Gelatin hydrolysis
  • Starch formulation
  • Glucose
  • Galactose
  • L-Sorbose
  • Sucrose
  • Maltose
  • Cellobiose
  • Trehalose
  • Lactose
  • Melibiose
  • Raffinose
  • Melezitose
  • Inulin
  • Soluble Starch
  • d-Xylose
  • L-Arabinose
  • D-Arabinose
  • L-Rhamnose
  • N-Acetyl-D-Glucosamine
  • Methanol
  • Ethanol
  • Glycerol
  • Erythritol
  • D-Mannitol
  • Alpha-Methyl-D-Glucocide
  • Salicin
  • D-Gluconate
  • Citrate
  • Inositol
  • Nitrate
  • 10%Nacl5%glucose
  • Starch formation
  • Gelatin Liquefacation
  • Urease
  • Growth at 30 degree C
  • Growth at 37 degree C
 

FAME Analysis .

FAME (Fatty Acid Methyl Ester) analysis is a phenotypic approach and the only commercially available microbial identification system that uses Cellular Fatty Analysis by Gas Chromatography (GC-FAME).

Researchers have identified microbes using gas chromatography analysis of cellular fatty acids (GC-FAME) for half a century. For 25 of those years, so has the Sherlock MIS. In fact, there is no other commercially-available identification system today that has been able to harness this reliable, time-tested technology. It includes

FAME Analysis of:

  • Aerobic bacteria
  • Anaerobic bacteria
  • Yeast culture
  • Fungi

This analysis can be used in the following range for identification:

  • Pharma
  • Agriculture Research
  • Animal Science
  • Biodefence
  • Bioremediation
  • Clinical Diagnosis
  • Marine Science
  • Medical Microbiology
  • Plant Pathology
  • Soil Science and other areas where microbial identification is important

 

Edible Oil Testing .

Oils acts as a vehicle to carry the soluble vitamins, nutrients, anti-oxidants to the body.

On the basis of these recommendations there are 3 parameters to adjudge any oil as healthy oil.

  • Ratio of saturated/mono saturated/polyunsaturated fatty acid ,ratio of essential fatty acids (omega6/omega3) and presence of natural antioxidants.
  • It is now widely accepted that what the human body needs is a ratio varying from 5:1 to 10:1 of omega 6 to omega 3.
  • The ratio of polyunsaturated fatty acid (PUFA) and saturated fatty acids (SFA) should be between 0.8 to 1.0

We provide service of measuring physio chemical properties of oils:

  • Iodine value
  • Acid Value
  • Saponification
  • Peroxide Value
  • Detecting of Carbon atoms and fatty acid analysis of oils

 

Quantification of Biomolecules .

Accurate quantification of biomolecules is important in the design of new biomaterials, diagnostic devices and sensors, as well as for the understanding of biomolecular interactions.

We provide the Quantification of :

  • Proteins
  • Carbohydrates
  • Polar Lipids
  • Neutral Lipids
  • Glyco Lipids

 

Genomic Services .

We are Importer & Distributor of the following Genomic Services:

  1. 18S rDNA sequencing of fungi
  2. 16S rDNA sequencing of Bacteria
  3. 16S rDNA sequencing of Actinomycetes / Yeast
  4. Sequencing of Virus only if PCR product is the given sample
  5. Primer Sequencing above 1.5kb single strand per bp
  6. Purified plasmid(single strand) primer walking of 4.8kb
  7. G+C Content
  8. Phylogenic tree analysis
  9. 16s rDNA if PCR product is given sample 500-1500 bp ( repeated reaction)
  10. Algae identification (400-800bp fragment of 16srDNA)
  11. Yeast identification (sequence polymorphism in 2 regions of rDNA gene)
  12. Molecular analysis of total genomic DNA through RAPD and RFLP
  13. Detection of Hepatitis C Virus (HCV)

 

Microbial Screening .

Our Microbial Screening And Miscellaneous Services are as follows:

  1. Enumeration: Total viable count (Total Coliforms / E.coli / Enterobacteriaceae / Staph. aureus)
  2. Staining: Gram positive / Gram negative(Bacillus / Yeast and Mold / Anaerobes)
  3. Pathogen Screening: Salmonella / E.coli 0157 / Listeria / Listeria monocytogenes / Campylobacter/ Staph enterotoxins
  4. Sterility Testing: Aerobic / Anaerobic / Bacteria and Fungi / Lactic acid bacteria
  5. Complete Analysis of Water
  6. Bioburden
  7. Polyphasic Report match with fame & DNA on DNA Sherlock Software

 

Phylogenetic Analysis .

In addition to microbial identification by fatty acid methyl ester (FAME) analysis, the Sherlock Software can also be used to identify microbes based on 16S/28S rRNA gene sequencing. Sherlock DNA begins where the DNA sequencer leaves off: with a textual consensus sequence. The advantage of this approach is that Sherlock DNA can be used with any sequencer from any vendor.

16S identification Neighbor Joining Tree Software is a static technique, giving a constant result regardless of growth conditions. Further, 16S is extremely reproducible, with only slight run-to-run variation. The 16S gene is sequenced because it is highly conserved at the species level. Thus, different strains of the same species will have identical or nearly identical results.

We offer a wide range of services related to DNA Sequencing. DNA Sequencing is a processes of evaluation of the nucleotide bases—adenine, cytosine, guanine and thymine—in a molecule of DNA. DNA sequence is very important for basic biological researches and is required in various areas like diagnostic, biotechnology or forensic biology. Number of DNA sequencing technologies has been developed to perform sequencing. Our professionals use FAME analysis, DNA sequencing and microscopic analysis for identifications, which make us the preferred choice of many clients. The mentioned tools are combined to form a well-rounded and exact identification system.

While 16S is more reliable for some species-level identification, when one desires strain-level information as well, FAME could be a better choice. And for specific genera, one technique or the other may be found to yield better results.

For even more information about a sample, one can use the Sherlock Software to process both FAME and 16S identifications and correlate the results in a single "polyphasic" report. Given that FAME and 16S are orthogonal techniques (techniques that rely on independent information from the samples), when the two techniques agree, the overall confidence in the result is greatly improved.

The Sherlock DNA Analysis mode allows for samples to be compared directly to one another, and results can be displayed in a number of forms. These forms include the familiar Neighbor Joining trees and Sequence Alignment tools. Further, MIDI has developed a new display type, the Accurate Root Neighbor Joining tree, which is specially designed for comparing an unknown sample to a group of known samples.

Key Features of Sherlock DNA include:

  • Validated libraries for bacterial ID (500bp and full gene 16S).
  • Validated libraries for fungi/yeast ID (500bp 28S).
  • Full integration with MIDI's Sherlock Software.
  • Combined reporting of DNA-FAME results.
  • DNA analysis tools for direct comparison of DNA samples.
  • Custom DNA library development tools.
  • Any DNA sequencer vendor can be used.

 

Complete Soil Analysis .

Soil bacteria and fungi play pivotal roles in various biogeochemical cycles and are responsible for the cycling of organic compounds.Soil microorganisms also influence above-ground ecosystems by contributing to plant nutrition, plant health, soil structure and soil fertility.

We provide the following services:

  1. Analysis of Microbial diversity in Soil
  2. C- analysis of soil
  3. Quantification of biomass in the soil